Differential chain-length specificities of two isoamylase-type starch-debranching enzymes from developing seeds of kidney bean.
نویسندگان
چکیده
Plant isoamylase-type starch-debranching enzymes (ISAs) hydrolyze alpha-1,6-linkages in alpha-1,4/alpha-1,6-linked polyglucans. Two ISAs, designated PvISA1/2 and PvISA3, were purified from developing seeds of kidney bean by ammonium sulfate fractionation and several column chromatographic procedures. The enzymes displayed different substrate specificities for polyglucans: PvISA1/2 showed broad chain-length specificities, whereas PvISA3 liberated specific chains with a DP of 2 to 4.
منابع مشابه
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ورودعنوان ژورنال:
- Bioscience, biotechnology, and biochemistry
دوره 71 9 شماره
صفحات -
تاریخ انتشار 2007